Research Lab Technician position available in Vierstra Lab

This position is full-time and works approximately 37.5 hours per week. This position is in the Department of Biology.  This position is for the Danforth Campus.

Position Summary: The objective of our research is to understand how the collection of red/far-red light-absorbing photochromic photoreceptors - phytochromes - enable perception of light by plants and microorganisms through analysis of their 3D structures using atomic level methods such as x-ray crystallography and cryo-electron microscopy.  The advertised position will aid in these efforts by expressing and purifying recombinant wild-type and mutant versions of these photoreceptors, assist in their biophysical and biochemical analyses, and help develop phytochrome samples for subsequent structural studies.

Primary Duties and Responsibilities:

  1. Express recombinant phytochromes in Escherichia coli-based synthesis systems.
  2. Purify recombinant phytochromes using various chromatography approaches, including FPLC.
  3. Define the purity of the phytochrome preparations via UV-visible absorption spectroscopy and SDS-PAGE.
  4. Analyze the photoconversion and thermal reversion kinetics of the phytochrome preparations via spectroscopic assays.
  5. Generate mutant forms of phytochromes by site-directed mutagenic strategies for subsequent purification and analysis as above.
  6. Help with laboratory maintenance and ordering of supplies.
  7. Perform other duties as assigned.

Required Qualifications:

B.S degree in biology, biochemistry, or related areas and 1 year of experience or equivalent education/experience to equal 5 years.

Preferred Qualifications:

  • Prior research experience helpful, especially related to microbiology and bacteria growth.
  • Excellent technical and communication skills helpful

Candidates can apply by navigating to https://jobs.wustl.edu/ and searching for the job opening number 45216.