This position is full-time and works approximately 37.5 hours per week. This position is in the Department of Biology. This position is for the Danforth Campus.
Position Summary: The objective of our research is to understand how the collection of red/far-red light-absorbing photochromic photoreceptors - phytochromes - enable perception of light by plants and microorganisms through analysis of their 3D structures using atomic level methods such as x-ray crystallography and cryo-electron microscopy. The advertised position will aid in these efforts by expressing and purifying recombinant wild-type and mutant versions of these photoreceptors, assist in their biophysical and biochemical analyses, and help develop phytochrome samples for subsequent structural studies.
Primary Duties and Responsibilities:
- Express recombinant phytochromes in Escherichia coli-based synthesis systems.
- Purify recombinant phytochromes using various chromatography approaches, including FPLC.
- Define the purity of the phytochrome preparations via UV-visible absorption spectroscopy and SDS-PAGE.
- Analyze the photoconversion and thermal reversion kinetics of the phytochrome preparations via spectroscopic assays.
- Generate mutant forms of phytochromes by site-directed mutagenic strategies for subsequent purification and analysis as above.
- Help with laboratory maintenance and ordering of supplies.
- Perform other duties as assigned.
B.S degree in biology, biochemistry, or related areas and 1 year of experience or equivalent education/experience to equal 5 years.
- Prior research experience helpful, especially related to microbiology and bacteria growth.
- Excellent technical and communication skills helpful
Candidates can apply by navigating to https://jobs.wustl.edu/ and searching for the job opening number 45216.