The majority of the laboratory work focuses on a research question using biochemical methods. The course consists of three sections: protein biochemistry, mass spectrometry, and structural biology. In the first section of the course, students learn principles and methods for heterologous expression of proteins in bacteria, protein quantitation, protein purification, SDS-PAGE, and kinetic analysis of enzyme activity. The second part focuses on using mass spectrometry to identify and to characterize proteins. The final part of the course introduces students to concepts of structural biology including protein crystallization, x-ray diffraction, and computer modeling of protein structures. Fulfills the upper-level laboratory requirement for the Biology major. Prerequisites: Chem 262 and either Bio 451 or Bio 4810/Chem 481. Suggested to be taken concurrently with Bio4820/Chem482.
Course Attributes: FA NSMBU SCIAR NSMAS NSM
Section 01Laboratory in Protein Analysis, Proteomics, and Protein Structure
INSTRUCTOR: CruzView Course Listing